Decontamination of oil-polluted soils: Power of electronic bioinformatic databases

The main idea was to solve the problem related to oil contamination of soil using bioremediation with bioaugmentation with modeled microorganism strains. The paper aimed to develop a bacterial consortium for petroleum hydrocarbon degradation during the biological treatment of oil-contaminated soils using electronic databases. The research methodology included an analysis of the mechanisms and metabolic pathways of petroleum hydrocarbon degradation and an assessment of the possible reaction modulus and enzymatic systems for the degradation of aromatic compounds. The taxonomic classification and review of oil compound transformation metabolic pathways were carried out using electronic KEGG, MetaCyc, and EzTaxon databases. The KEGG database was used to create a microbiological consortium of certain strains of bacteria that improved hydrocarbon degradation process performance. Identification of bacteria’s complete genome using Island Viewer 4 allowed to create of a consortium of oil-destructive bacteria consisting of such strains: Pseudoxanthomonas spadix BD-a59, Rhodococcus jostii RHA1, Rhodococcus aetherivorans IcdP1, Pseudomonas putida ND6, Pseudomonas stutzeri 19SMN4, Pseudomonas fluorescens UK4, Acinetobacter lactucae OTEC-02, Bacillus cereus F837/76.7.9. The ratio between the mentioned strains of microorganisms in the consortium was set at 20 % : 20 % : 15 % : 10 % : 10 % : 5 % : 5 % : 15 %. This bacterial consortium for aromatic hydrocarbons was created according to the metabolic information of basic enzymatic systems and the predominant transformation of particular oil compounds using the BacDive database

Спосіб розділення відходів буріння нафтових свердловин з подальшою утилізацією окремих фракцій

Спосіб розділення відходів буріння нафтових свердловин з подальшою утилізацією окремих фракцій включає збирання відходів буріння, регулювання рН та обробку коагулянтами і флокулянтами, розділення відходів на тверду і рідку фази з наступним використанням рідкої фази та утилізацією твердої фази відходів. При розділенні відходів буріння на фази, рідка фаза розділяється на нафвідтову і водну складові

Technologies for Environmental Safety Application of Digestate as Biofertilizer

The purpose of the paper is to determine the environmentally safe and economically feasible technology of biofertilizer production from the digestate including dewatering process. Methodological basis is based on the systematic approach to the determination of factors effected on the distribution of nutrients and pollutants between liquid and solid fractions after digestate separation. The paper analyzes modern technologies aimed at dewatering the digestate and reduction of its volume, showed their effectiveness. These technologies allow expanding the opportunities for commercialization of the digestate, increasing the cost of its transportation and application to the soil instead of complex fertilizers, using some valuable products. The results of the study showed that the ecological quality of the digestate is the highest as well as co-digested thermally pre-treated feedstock is used for solid-liquid separation in centrifuge with polymer addition as post-treatment approach to the flocculation. In order to increase efficiency of biofertilizer application the technological scheme of production process of granular fertilizers from digestate was proposed. Special feature of this scheme is in the use of phosphogypsum binder for the production of organo-mineral fertilizer that contributes phosphogypsum recycling in the waste management system